Journal: Journal of Lipid Research

Article Title: Hepatoprotective drug screening identifies daclatasvir, a promising therapeutic candidate for MASLD by targeting PLIN2

doi: 10.1016/j.jlr.2025.100835

Figure Lengend Snippet: Daclatasvir treatment ameliorates liver damage and fibrosis in MCD diet-induced MASH mouse model. A: Schematic illustration of the experimental workflow for the daclatasvir treatment strategy in a methionine-choline deficient (MCD) diet-induced MASH mouse model. B: Liver weight, LW/BW, and LW/BMI were recorded for MCD diet-fed mice after 3 weeks of treatment with either vehicle or daclatasvir. C: Representative images of H&E, PSR, and CD11b staining of liver samples from vehicle or daclatasvir treated mice at 4 weeks of MCD diet administration. Scale bar, 200 μm for H&E and PSR; 100 μm for CD11b. D: Quantitative results for PSR and CD11b staining shown in (C). E: Enzyme activities of serum hepatic transaminases in MCD diet-fed mice after 3 weeks of treatment with either vehicle or daclatasvir. F–H: The mRNA expression of genes associated with lipid catabolism (F), inflammation (G), and fibrosis (H) in liver tissues from MCD diet-induced MASH mice following 3 weeks of daclatasvir or vehicle treatment. For (B–H), n = 6 mice per group. Data are presented as mean ± SD; ∗ P < 0.05, ∗∗ P < 0.01; Student’s t test for statistics.

Article Snippet: The sections were incubated with a rabbit anti-CD11b primary antibody (BM3925, Boster) overnight at 4°C, followed by incubation with a goat anti-rabbit fluorophore-conjugated secondary antibody (A-11036, Invitrogen).

Techniques: Staining, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

doi: 10.1111/jcmm.18294

Figure Lengend Snippet: The expression of forkhead box protein 1 (FOXP1) in oesophageal cancer and various tumours. (A) The expression of FOXP1 in various tumours in GEPIA database. (B) The expression of FOXP1 in oesophageal cancer tissues and normal tissues in UALCAN database. (C–E) The expression of FOXP1 in oesophageal cancer tissues depending on pathological type, tumour grade and tumour stage. (F–I) The expression of FOXP1 in oesophageal squamous cell carcinoma in GSE17351, GSE70409, GSE77861 and GSE100942 datasets. (J–K) The expression of FOXP1 in oesophageal squamous cell carcinoma tissues and adjacent normal tissues. * p < 0.05.

Article Snippet: FOXP1 primary antibody (1:150 dilution, BOSTER, M00723‐4) was added and incubated at room temperature for 1 h. The slices were incubated with the secondary antibody for 30 min at room temperature after washing with PBS buffer.

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

doi: 10.1111/jcmm.18294

Figure Lengend Snippet: High expression of forkhead box protein 1 (FOXP1) indicates a better survival prognosis in oesophageal cancer. (A) The correlation between expression of FOXP1 and overall survival prognosis in different tumours in TISCH2 database. (B) Kaplan–Meier survival curves of overall survival for oesophageal cancer patients with high and low expression of FOXP1 in GEPIA database. (C) Kaplan–Meier survival curves of disease free survival for oesophageal cancer patients with high and low expression of FOXP1 in GEPIA database. (D) Kaplan–Meier survival curves of overall survival for oesophageal cancer patients with high and low expression of FOXP1 in KM plotter database. (E) Kaplan–Meier survival curves of disease free survival for oesophageal cancer patients with high and low expression of FOXP1 in KM plotter database. (F) Kaplan–Meier survival curves of overall survival for oesophageal squamous cell carcinoma patients with high and low expression of FOXP1 in KM plotter database. (G) Kaplan–Meier survival curves of overall survival for oesophageal squamous cell carcinoma patients with high and low expression of FOXP1 in GSE53625 dataset.

Article Snippet: FOXP1 primary antibody (1:150 dilution, BOSTER, M00723‐4) was added and incubated at room temperature for 1 h. The slices were incubated with the secondary antibody for 30 min at room temperature after washing with PBS buffer.

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

doi: 10.1111/jcmm.18294

Figure Lengend Snippet: Identification of differential expression genes in high and low expression of forkhead box protein 1 (FOXP1) cohorts in oesophageal squamous cell carcinoma. (A) Venn diagram showing the intersection of differential expression genes in GSE26886 and GSE45168 datasets. (B, C) Volcano plots showing the differential expression genes in GSE26886 and GSE45168 datasets. (D, E) Cluster heatmaps of genes in GSE26886 and GSE45168 datasets.

Article Snippet: FOXP1 primary antibody (1:150 dilution, BOSTER, M00723‐4) was added and incubated at room temperature for 1 h. The slices were incubated with the secondary antibody for 30 min at room temperature after washing with PBS buffer.

Techniques: Quantitative Proteomics, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

doi: 10.1111/jcmm.18294

Figure Lengend Snippet: Forkhead box protein 1 (FOXP1) functional analysis and interaction network construction. (A) Gene ontology enrichment analysis for differential expression genes. (B) KEGG enrichment analysis for differential expression genes. (C) Network of enriched terms in metascape database. (D) The cord diagram of the correspondence between differential expression genes and signalling pathways. (E, F) Protein–protein interaction (PPI) network and MCODE components identified in differential expression genes in Metascape database. (G) The gene co‐expression network for FOXP1 in GeneMANIA database. (H) PPI network of differential expression genes in STRING database.

Article Snippet: FOXP1 primary antibody (1:150 dilution, BOSTER, M00723‐4) was added and incubated at room temperature for 1 h. The slices were incubated with the secondary antibody for 30 min at room temperature after washing with PBS buffer.

Techniques: Functional Assay, Quantitative Proteomics, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

doi: 10.1111/jcmm.18294

Figure Lengend Snippet: Association of forkhead box protein 1 (FOXP1) with the tumour immune microenvironment in oesophageal cancer. (A) The association of FOXP1 expression and tumour immune infiltration cells level in oesophageal cancer in TIMER database. (B) The relative proportion of different somatic copy number alterations (SCNA) states of FOXP1 for various TCGA tumour types in TIMER2.0 database. (C) The relationship between different SCNA states of FOXP1 and infiltration level of six types of immune cells in oesophageal cancer. * p < 0.05. (D) The relative proportion of the infiltration levels of 22 immune infiltrating cells in oesophageal squamous cell carcinoma based on GSE75241 dataset. (E) The immune cells correlation heatmap in oesophageal squamous cell carcinoma based on GSE75241 dataset.

Article Snippet: FOXP1 primary antibody (1:150 dilution, BOSTER, M00723‐4) was added and incubated at room temperature for 1 h. The slices were incubated with the secondary antibody for 30 min at room temperature after washing with PBS buffer.

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: An integrated analysis of the anticarcinogenic role of forkhead box protein 1 in oesophageal squamous cell carcinoma

doi: 10.1111/jcmm.18294

Figure Lengend Snippet: Forkhead box protein 1 (FOXP1) inhibits proliferation of oesophageal squamous cell carcinoma cells. (A, B) Western blot assay confirmed the expression levels of FOXP1 protein in different oesophageal squamous cell carcinoma cell lines and oesophageal epithelial cells. * p < 0.05, ** p < 0.01, ns p > 0.05. (C, D) Western blot assay confirmed the transfection efficiency of overexpression of FOXP1 in EC109 cells. ** p < 0.01. (E) The CCK‐8 assay showed overexpression of FOXP1 inhibits the proliferation of EC109 cells. *** p < 0.001. (F, G) The colony formation assay showed overexpression of FOXP1 inhibits the colony forming ability of EC109 cells. (H, I) The subcutaneous tumour formation assay showed overexpression of FOXP1 inhibits the ability of tumorigenicity of EC109 cells in vivo. * p < 0.05, ** p < 0.01, ns p > 0.05.

Article Snippet: FOXP1 primary antibody (1:150 dilution, BOSTER, M00723‐4) was added and incubated at room temperature for 1 h. The slices were incubated with the secondary antibody for 30 min at room temperature after washing with PBS buffer.

Techniques: Western Blot, Expressing, Transfection, Over Expression, CCK-8 Assay, Colony Assay, Tube Formation Assay, In Vivo